Human GRB2 (SH2 Domain) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB38461

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB38461
MAB38461-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 669604

View all GRB2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRB2 (SH2 Domain)
Trp60-Glu152
Accession # P62993

Specificity

Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GRB2 (SH2 Domain) Antibody

Detection of Human, Mouse, and Rat GRB2 antibody by Western Blot.

Detection of Human, Mouse, and Rat GRB2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRB2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GRB2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

GRB2 in Human Breast Cancer Tissue.

GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GRB2 (SH2 Domain) Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB38461 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRB2

GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules.  It serves as a linker for many intracellular proteins.  Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors.  Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation.  Human GRB2 is 217 amino acids (aa) in length.  It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212).  SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions.  There is one splice form that shows a deletion of aa 60-100.  This removes the SH2 domain and initiates apoptosis.  Human GRB2 is over 99% aa identical to mouse and dog GRB2.

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Entrez Gene IDs

2885 (Human); 14784 (Mouse); 81504 (Rat)

Gene Symbol

GRB2

UniProt

P62993

Additional GRB2 Products

Product Documents for Human GRB2 (SH2 Domain) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GRB2 (SH2 Domain) Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.