Human GRB2 (SH2 Domain) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB38461
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 669604
Product Specifications
Immunogen
E. coli-derived recombinant human GRB2 (SH2 Domain)
Trp60-Glu152
Accession # P62993
Trp60-Glu152
Accession # P62993
Specificity
Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GRB2 (SH2 Domain) Antibody
Detection of Human, Mouse, and Rat GRB2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRB2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GRB2 in Human Breast Cancer Tissue.
GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GRB2 (SH2 Domain) Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Sample: HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB38461 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRB2
Long Name
Growth Factor Receptor-Bound Protein 2
Alternate Names
ASH, EGFRBP-GRB2, Grb3-3
Gene Symbol
GRB2
UniProt
Additional GRB2 Products
Product Documents for Human GRB2 (SH2 Domain) Antibody
Product Specific Notices for Human GRB2 (SH2 Domain) Antibody
For research use only
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