Human GRK5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4539
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 691816
Product Specifications
Immunogen
E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947
Pro463-Ser590
Accession # P34947
Specificity
Detects human GRK5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity
with recombinant human (rh) GRK6 is observed and no cross-reactivity with rhGRK4
is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GRK5 Antibody
GRK5 in BJAB Human Cell Line.
GRK5 was detected in immersion fixed BJAB human Burkitt's lymphoma cell line using Mouse Anti-Human GRK5 Monoclonal Antibody (Catalog # MAB4539) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human GRK5 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BJAB human Burkitt's lymphoma cell line
Sample: Immersion fixed BJAB human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRK5
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.
Long Name
G protein-coupled Receptor Kinase 5
Alternate Names
GPRK5
Gene Symbol
GRK5
UniProt
Additional GRK5 Products
Product Documents for Human GRK5 Antibody
Product Specific Notices for Human GRK5 Antibody
For research use only
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