Human GSK-3 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB25061
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 272535
Product Specifications
Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Met1-Thr420
Accession # P49841
Specificity
Detects human GSK-3 beta.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human GSK-3 beta Antibody
GSK-3 beta in MCF-7 Human Cell Line.
GSK-3 beta was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GSK-3 beta Monoclonal Antibody (Catalog # MAB25061) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GSK-3 beta Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GSK-3 beta
Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved inenergy metabolism, body pattern formation, and neuronal cell development.
Long Name
Glycogen Synthase Kinase 3 beta
Alternate Names
GSK3 beta, GSK3B
Gene Symbol
GSK3B
UniProt
Additional GSK-3 beta Products
Product Documents for Human GSK-3 beta Antibody
Product Specific Notices for Human GSK-3 beta Antibody
For research use only
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