Human Guanylyl Cyclase C/GUCY2C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2157

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2543C

View all Guanylyl Cyclase C/GUCY2C Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
Ser21-Gln430
Accession # P25092

Specificity

Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Guanylyl Cyclase C/GUCY2C Antibody

Detection of GUCY2C antibody in HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP antibody by Flow Cytometry.

Detection of GUCY2C in HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human GUCY2C or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human GUCY2C Monoclonal Antibody (Catalog # MAB2157) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Guanylyl Cyclase C/GUCY2C antibody in LS1034 Human Cell Line by Immunocytochemistry (ICC).

Guanylyl Cyclase C/GUCY2C in LS1034 Human Cell Line.

Guanylyl Cyclase C/GUCY2C was detected in immersion fixed LS1034 human colorectal carcinoma cell line (positive) and HCT-116 human colorectal carcinoma cell line (negative) using Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C Monoclonal Antibody (Catalog # MAB2157) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Guanylyl Cyclase C/GUCY2C Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human GUCY2C and eGFP 

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed LS1034 human colorectal carcinoma cell line (positive) and HCT‑116 human colorectal carcinoma cell line (negative) 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Human Guanylyl Cyclase C/GUCY2C Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Guanylyl Cyclase C/GUCY2C Antibody

For research use only

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