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Human HAI-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM10482

R&D Systems, part of Bio-Techne
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BAM10482

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 169428

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278

Specificity

Detects human HAI-1 In ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse HAI-1, recombinant human (rh) TFPI, rhTFPI-2, rhAPP, rhHAI-2A, rhGASP-1, or rhGASP-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human HAI-1 Biotinylated Antibody

Human HAI-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human HAI-1 Antibody (Catalog # MAB10481)
  • Standard: Recombinant Human HAI-1 Protein, CF (Catalog # 1048-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References

  1. Denda, K. et al. (2002) J. Biol. Chem. 277:14053. 
  2. Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Long Name

HGF Activator Inhibitor Type 1

Alternate Names

HAI1, SPINT1

Entrez Gene IDs

6692 (Human); 20732 (Mouse)

Gene Symbol

SPINT1

UniProt

Additional HAI-1 Products

Product Documents for Human HAI-1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HAI-1 Biotinylated Antibody

For research use only

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