Human HAI-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1106
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-2A
Ala28-Lys197
Accession # O43291
Ala28-Lys197
Accession # O43291
Specificity
Detects human HAI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human HAI‑1 and recombinant mouse HAI-2B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human HAI-2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HAI‑2A (Catalog # 1106‑PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human HAI‑2A (Catalog # 1106‑PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human HAI-2A (Catalog # 1106-PI)
Sample: Recombinant Human HAI-2A (Catalog # 1106-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-2
Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1-5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.
References
- Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
- Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
- Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
- Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
- Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
Long Name
HGF Activator Inhibitor Type 2
Alternate Names
HAI2
Gene Symbol
SPINT2
UniProt
Additional HAI-2 Products
Product Documents for Human HAI-2 Antibody
Product Specific Notices for Human HAI-2 Antibody
For research use only
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