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Human HAI‑2 Ectodomain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1106

R&D Systems, part of Bio-Techne
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MAB1106
MAB1106-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 170018

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-2
aa 28-197
Accession # O43291

Specificity

Detects recombinant human (rh)HAI-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhHAI-1 or rmHAI-2B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human HAI‑2 Ectodomain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human HAI‑2 (Catalog # 1106-PI)

Human HAI-2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human HAI-2 Biotinylated Antibody (Catalog # BAF1106)
  • Standard: Recombinant Human HAI-2A Protein, CF (Catalog # 1106-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using MAB1106 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

HGF Activator Inhibitor type II (HAI-2), also known as placental bikunin, is a Kunitz-type protease inhibitor. It is a type I membrane protein containing 2 Kunitz inhibitor domains in its extracellular region. In mouse, at least three isoforms of HAI-2 exist as a result of alternative splicing. Isoforms 2 and 3, also known as HAI-2B and HAI-2C, respectively, are both missing the first amino-terminal Kunitz inhibitor domain.

Long Name

HGF Activator Inhibitor Type 2

Alternate Names

HAI2

Entrez Gene IDs

10653 (Human); 20733 (Mouse)

Gene Symbol

SPINT2

UniProt

Additional HAI-2 Products

Product Documents for Human HAI‑2 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HAI‑2 Ectodomain Antibody

For research use only

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