Human HAND1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3168
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004
Met1-Gln215
Accession # O96004
Specificity
Detects human human HAND1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human HAND1 Biotinylated Antibody
HAND1 in SH-SY5Y Human Cell Line.
HAND1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3168) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human HAND1 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human HAND1
Sample: Recombinant Human HAND1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAND1
Long Name
Heart And Neural Crest Derivatives Expressed 1
Alternate Names
eHand, Thing1
Gene Symbol
HAND1
UniProt
Additional HAND1 Products
Product Documents for Human HAND1 Biotinylated Antibody
Product Specific Notices for Human HAND1 Biotinylated Antibody
For research use only
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