Human HCN4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8138
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HCN4
Lys1084-Leu1203
Accession # Q9Y3Q4
Lys1084-Leu1203
Accession # Q9Y3Q4
Specificity
Detects human HCN4 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human HCN4 Antibody
HCN4 in BG01V Human Embyonic Stem Cells.
HCN4 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into cardiomyocytes (upper panel), using Sheep Anti-Human HCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human HCN4 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes
Reviewed Applications
Read 1 review rated 5 using AF8138 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HCN4
HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.
Long Name
Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4
Alternate Names
SSS2
Gene Symbol
HCN4
UniProt
Additional HCN4 Products
Product Documents for Human HCN4 Antibody
Product Specific Notices for Human HCN4 Antibody
For research use only
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