Human Helios PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC73092P

R&D Systems, part of Bio-Techne
Discontinued Product
IC73092P has been discontinued. View all Helios products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 736440

View all Helios Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Helios
Met1-Gln97
Accession # Q9UKS7

Specificity

Detects human Helios in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Helios PE-conjugated Antibody

Detection of Helios antibody in Human PBMCs Stimulated to Induce Tregs antibody by Flow Cytometry.

Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) overnight were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8214R) and either (A) Mouse Anti-Human Helios PE-conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Helios antibody in Human PBMCs Stimulated to Induce Tregs antibody by Flow Cytometry.

Detection of Helios in Human PBMCs Stimulated to Induce Tregs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) overnight were stained with Mouse Anti-Human CD25/IL-2 R alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1020G) and either (A) Mouse Anti-Human Helios PE-conjugated Monoclonal Antibody (Catalog # IC73092P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Helios PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Helios

Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (aa 112-219) and two C-terminal zinc finger domains (aa 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within aa 1-97, human and mouse Helios share 96% aa sequence identity.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Entrez Gene IDs

22807 (Human); 22779 (Mouse)

Gene Symbol

IKZF2

UniProt

Q9UKS7 (Human)

Additional Helios Products

Product Documents for Human Helios PE-conjugated Antibody

Product Datasheets

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Product Specific Notices for Human Helios PE-conjugated Antibody

For research use only

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