Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5968

R&D Systems, part of Bio-Techne
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AF5968
AF5968-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human HS3ST1
Arg21-His307
Accession # O14792

Specificity

Detects human HS3ST1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Detection of Human HS3ST1 antibody by Western Blot.

Detection of Human HS3ST1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human HS3ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS3ST1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HS3ST1 (Catalog # 5968-ST), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1

Heparan sulfate is a highly sulfated polysaccharide that can be found on cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is detached from the protein core and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑-acetylglucosamine residues can be sulfated at N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST1 is a rate-limiting enzyme for generating an antithrombin-binding pentasaccharide epitope on heparan sulfate and heparin (5, 6). Unlike other sulfotransferases that have signal-anchor domains and are type II membrane integral proteins in Golgi apparatus, HS3ST1 lacks a transmembrane domain and is likely to be an intraluminal enzyme (7, 8).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Rosenberg, R.D. et al. (1997) J. Clin. Invest. 100:S67.
  6. Esko, J.D and Lindahl, U. (2001) J. Clin. Invest. 108:169.
  7. Shworak, N.W. et al. (1997) J. Biol. Chem. 272:28008.
  8. Liu, J. et al. (1999) J. Biol. Chem. 274:5158.

Alternate Names

3OST1, h3-OST-1

Entrez Gene IDs

9957 (Human); 15476 (Mouse); 84406 (Rat)

Gene Symbol

HS3ST1

UniProt

O14792

Additional Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Products

Product Documents for Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

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