Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6085

R&D Systems, part of Bio-Techne
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MAB6085
MAB6085-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 712010

View all Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4
Gly184-Lys456
Accession # Q9Y661

Specificity

Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HS3ST3B1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 antibody in Human Brain by Immunohistochemistry (IHC-P).

Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 in Human Brain.

Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB6085) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HS3ST4 (Catalog # 6085‑ST), see our available Western blot detection antibodies

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
  6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
  7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
  8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.

Alternate Names

3-OST-4

Entrez Gene IDs

9951 (Human)

Gene Symbol

HS3ST4

UniProt

Q9Y661

Additional Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Products

Product Documents for Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

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