Human Hexim 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8106
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Hexim 1
His213-Asp359
Accession # O94992
His213-Asp359
Accession # O94992
Specificity
Detects human Hexim 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Hexim 2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Hexim 1 Antibody
Detection of Human Hexim 1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexim 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hexim 1 in Human Liver.
Hexim 1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Hexim 1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and Hep3B human hepatocellular carcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and Hep3B human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hexim 1
Long Name
Hexamethylene Bis-Acetamide Inducible 1
Alternate Names
CLP1, HEXIM1, HIS1, MAQ1
Gene Symbol
HEXIM1
UniProt
Additional Hexim 1 Products
Product Documents for Human Hexim 1 Antibody
Product Specific Notices for Human Hexim 1 Antibody
For research use only
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