U937 human histiocytic lymphoma cell line, resting (A) or (B) activated with PMA, was stained with Mouse Anti-Human Histamine H1 R PE-conjugated Monoclonal Antibody (Catalog # FAB4726P, filled histograms) or isotype control antibody (Catalog # IC002P, open histograms). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Histamine H1 R PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: U937 human histiocytic lymphoma cell line activated with PMA

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

Long Name

Histamine H1 Receptor

Alternate Names

H1-R, Histamine H1R, HRH1

Entrez Gene IDs

3269 (Human); 15465 (Mouse); 24448 (Rat)

Gene Symbol

HRH1

UniProt

AAH60802

Additional Histamine H1 R Products

Product Documents for Human Histamine H1 R PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Histamine H1 R PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Histamine H1 R PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4726P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Histamine H1 R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Histamine H1 R PE-conjugated Antibody

Detection of Histamine H1 R in U937 human histiocytic lymphoma cell line activated with PMA by Flow Cytometry.