Human HLA-E Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11547
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1079755
Product Specifications
Immunogen
HLA-E containing synthetic peptide
Accession # P13747
Accession # P13747
Specificity
Detects a synthetic peptide around aa 80 in Direct ELISA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human HLA-E Antibody
Detection of HLA-E in Human Tonsil.
HLA-E was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-E Monoclonal Antibody (Catalog # MAB11547) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human HLA-E Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HLA-E
References
- Sharpe HR, Bowyer G, Brackenridge S, Lambe T. HLA-E: exploiting pathogen-host interactions for vaccine development. Clin Exp Immunol. 2019 May;196(2):167-177. doi: 10.1111/cei.13292. Epub 2019 Apr 9. PMID: 30968409; PMCID: PMC6468186.
- Lin Z, Bashirova AA, Viard M, Garner L, Quastel M, Beiersdorfer M, Kasprzak WK, Akdag M, Yuki Y, Ojeda P, Das S, Andresson T, Naranbhai V, Horowitz A, McMichael AJ, Hoelzemer A, Gillespie GM, Garcia-Beltran WF, Carrington M. HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses. Nat Immunol. 2023 Jul;24(7):1087-1097. doi: 10.1038/s41590-023-01523-z. Epub 2023 Jun 1. PMID: 37264229; PMCID: PMC10690437.
- Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood. 2007 Apr 1;109(7):2806-14. doi: 10.1182/blood-2006-06-030213. PMID: 17179229.
Long Name
Major Histocompatibility Complex, Class I, E
Alternate Names
HLA-6.2, HLAE, QA1
Entrez Gene IDs
3133 (Human)
Gene Symbol
HLA-E
UniProt
Additional HLA-E Products
Product Documents for Human HLA-E Antibody
Product Specific Notices for Human HLA-E Antibody
For research use only
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