Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human HM74A/PUMA-G/GPR109A Antibody

Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.

Human peripheral blood neutrophils were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P. View our protocol for Staining Membrane-associated Proteins.

Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HM74A/PUMA-G/GPR109A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human whole blood monocytes and neutrophils and HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HM74A/PUMA-G/GPR109A

HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Alternate Names

GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG

Entrez Gene IDs

338442 (Human); 80885 (Mouse)

Gene Symbol

HCAR2

UniProt

Q8TDS4

Additional HM74A/PUMA-G/GPR109A Products

Product Documents for Human HM74A/PUMA-G/GPR109A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human HM74A/PUMA-G/GPR109A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human HM74A/PUMA-G/GPR109A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2760

Key Product Details

Species Reactivity

Validated:

Cited:

Applications