Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2760A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 245106
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4
Met1-Pro363
Accession # Q8TDS4
Specificity
Detects human HM74A/PUMA-G/GPR109A. Stains human HM74A/PUMA-G/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.
Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.Applications for Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood neutrophils and HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP
Sample: Human peripheral blood neutrophils and HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HM74A/PUMA-G/GPR109A
HM74A/GPR109A (also known as PUMA-G in mouse) is a 50-55 kDa 7-transmembrane protein member of a small subfamily within the GPCR #1 family. It is expressed on or in a wide variety of cells, including microglia, neutrophils, islet beta-cells, macrophages, adipocytes, retinal pigment epithelium and keratinocytes. Although HM74A is noted for its ability to bind niacin, it natural ligand is beta-hydroxybutyrate. Thus it may naturally act as a negative regulator of fatty acid release by fat, which is converted to ketone bodies by the liver, and which feedback on adipocytes. Full-length human HM74A shares 95% amino acid (aa) sequence identity with human HM74 and 81% aa sequence identity with mouse HM74A.
Long Name
G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A
Alternate Names
GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG
Gene Symbol
HCAR2
UniProt
Additional HM74A/PUMA-G/GPR109A Products
Product Documents for Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
Product Specific Notices for Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...