Human HNF-4 alpha/NR2A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4605
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Bioassay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HNF‑4 alpha/NR2A1
Val130-Ser330
Accession # P41235
Val130-Ser330
Accession # P41235
Specificity
Detects human HNF‑4 alpha/NR2A1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human HNF-4 alpha/NR2A1 Antibody
Detection of Human HNF-4 alpha/NR2A1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HNF‑4 alpha/NR2A1 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HNF-4a/NR2A1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human HNF-4 alpha/NR2A1 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HNF-4 alpha/NR2A1
HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.
Long Name
Hepatocyte Nuclear Factor-4, alpha
Alternate Names
HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14
Entrez Gene IDs
3172 (Human)
Gene Symbol
HNF4A
UniProt
Additional HNF-4 alpha/NR2A1 Products
Product Documents for Human HNF-4 alpha/NR2A1 Antibody
Product Specific Notices for Human HNF-4 alpha/NR2A1 Antibody
For research use only
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