Human HOXB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6318
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HOXB1
Met1-Thr192
Accession # P14653
Met1-Thr192
Accession # P14653
Specificity
Detects human HOXB1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HOXA1, rhHOXB4, and rhHOXA9 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human HOXB1 Antibody
HOXB1 in NTera‑2 Human Cell Line.
HOXB1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cells, treated with (upper panel) and without (lower panel) retinoic acid, using Sheep Anti-Human HOXB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6318) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human HOXB1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cells treated with retinoic acid
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cells treated with retinoic acid
Reviewed Applications
Read 1 review rated 4 using AF6318 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOXB1
HOXB1 (Homeobox protein B1; also HOX-2I) is a 32 kDa (predicted) member of the Antp homeobox family, labial subfamily of transcription factors. It is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures. Among these are the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Human HOXB1 is 301 amino acids (aa) in length. It contains an Antp-type hexapeptide (aa 179-184) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 203-262). There is one potential splice variant that shows a 40 aa substitution for aa 193-301. Over aa 1-192, human HOXB1 shares 82% aa identity with mouse HOXB1.
Long Name
Homeobox B1
Alternate Names
HOX2, HOX2I
Gene Symbol
HOXB1
UniProt
Additional HOXB1 Products
Product Documents for Human HOXB1 Antibody
Product Specific Notices for Human HOXB1 Antibody
For research use only
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