Human HTRA1/PRSS11 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29161
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 275603
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HTRA1/PRSS11
Gln23-Pro480
Accession # Q92743
Gln23-Pro480
Accession # Q92743
Specificity
Detects human HTRA1/PRSS11 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human HTRA1/PRSS11 Antibody
HTRA1/PRSS11 in Human Hepatocellular Carcinoma and IBS Intestine.
HTRA1/PRSS11 was detected in immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine (positive staining; upper panels) and human cervical squamous cell carcinoma and smooth muscle (negative staining; lower panels) using Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB29161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane (luminal surface of intestinal gland). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human HTRA1/PRSS11 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine
Sample: Immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HTRA1/PRSS11
Long Name
High Temperature Requirement Protein-1
Alternate Names
IGFBP5-protease, PRSS11
Entrez Gene IDs
5654 (Human)
Gene Symbol
HTRA1
UniProt
Additional HTRA1/PRSS11 Products
Product Documents for Human HTRA1/PRSS11 Antibody
Product Specific Notices for Human HTRA1/PRSS11 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...