Human ICAM-1/CD54 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF720
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, ELISA Microarray Development, Flow Cytometry, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Extracellular domain
Specificity
Detects human ICAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhICAM-2 and rhICAM-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ICAM-1/CD54 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human ICAM-1/CD54 (Catalog # ADP4)
Sample: Recombinant Human ICAM-1/CD54 (Catalog # ADP4)
Human ICAM-1/CD54 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for Human ICAM-1/CD54 Biotinylated Antibody
Product Specific Notices for Human ICAM-1/CD54 Biotinylated Antibody
For research use only
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