Human ICAM-2/CD102 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF244
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Lys25-Gln223
Accession # CAA33630
Specificity
Clonality
Host
Isotype
Endotoxin Level
Scientific Data Images for Human ICAM-2/CD102 Antibody
Detection of Human ICAM-2/CD102 by Western Blot
ICAM-2 WT and variants co-precipitated with alpha-actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha-actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha-actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha-actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha-actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.Detection of Human ICAM-2/CD102 by Western Blot
ICAM-2 WT and variants co-precipitated with alpha-actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha-actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha-actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha-actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha-actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human ICAM-2/CD102 Antibody
Adhesion Blockade
CyTOF-ready
Flow Cytometry
Sample: Human peripheral blood monocytes
Western Blot
Sample: Recombinant Human ICAM-2/CD102 Fc Chimera (Catalog # 803-I2)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-2/CD102
Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.
References
- Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ICAM-2/CD102 Products
Product Documents for Human ICAM-2/CD102 Antibody
Product Specific Notices for Human ICAM-2/CD102 Antibody
For research use only