Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # MAB8131) or (B) Mouse IgG1 Isotype Control (MAB002), followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

ICAM-3/CD50 in Human Lymph Node.

ICAM-3/CD50 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # MAB8131) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human ICAM-3/CD50 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PBMC lymphocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha_d/ beta₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References

Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Entrez Gene IDs

3385 (Human)

Gene Symbol

ICAM3

UniProt

CAA49473

Additional ICAM-3/CD50 Products

Product Documents for Human ICAM-3/CD50 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ICAM-3/CD50 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ICAM-3/CD50 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8131

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all ICAM-3/CD50 Antibodies »

Product Summary for Human ICAM-3/CD50 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ICAM-3/CD50 Antibody

Detection of ICAM-3/CD50 in Human Blood Lymphocytes by Flow Cytometry.