Human ICOS APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6975A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 669222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8
Glu21-Phe141
Accession # Q9Y6W8
Specificity
Detects human ICOS in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ICOS APC-conjugated Antibody
Detection of ICOS in Human CD3+PBMCs by Flow Cytometry.
Human CD3+peripheral blood mononuclear cells (PBMCs) treated for 48 hours with 5 µg/mL PHA were stained with Mouse Anti-Human ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB6975A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human ICOS APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human CD3+ peripheral blood mononuclear cells (PBMCs) treated with PHA
Sample: Human CD3+ peripheral blood mononuclear cells (PBMCs) treated with PHA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICOS
Inducible Costimulator (ICOS), also known as AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule), CD278, and CRP-1 (CD28-Related Protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Over aa 21-141, human and mouse ICOS share approximately 70% aa sequence identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Additional ICOS Products
Product Documents for Human ICOS APC-conjugated Antibody
Product Specific Notices for Human ICOS APC-conjugated Antibody
For research use only
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