Human IFN-alpha / beta R1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF245
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730
Lys28-Lys436
Accession # AAA52730
Specificity
Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IFN-alpha / beta RI is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFN-alpha / beta R1 Antibody
Detection of Human IFN-alpha / beta R1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFN-alpha / beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IFN-alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human IFN-alpha / beta R1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Western Blot
0.1-1 µg/mL
Sample: Recombinant Human IFN-alpha / beta R1, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line
Sample: Recombinant Human IFN-alpha / beta R1, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha/beta R1
Type I interferons (IFN-alpha, IFN-beta, IFN-omega) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.
Long Name
Interferon alpha/beta Receptor 1
Alternate Names
IFN-aR1, IFN-bR1, IFNAR1, IFNbR1
Gene Symbol
IFNAR1
UniProt
Additional IFN-alpha/beta R1 Products
Product Documents for Human IFN-alpha / beta R1 Antibody
Product Specific Notices for Human IFN-alpha / beta R1 Antibody
Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.
For research use only
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