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Human IFN-alpha / beta R1 PerCP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB245C

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 85228

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730

Specificity

Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IFN-alpha / beta R1 PerCP-conjugated Antibody

Detection of IFN-a/ beta R1 antibody in Human U937 cell line antibody by Flow Cytometry.

Detection of IFN‑ alpha/ beta R1 in Human U937 cell line by Flow Cytometry.

Human U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human IFN-a/ beta R1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB245C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IFN-alpha / beta R1 PerCP-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

UniProt

Additional IFN-alpha/beta R1 Products

Product Documents for Human IFN-alpha / beta R1 PerCP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-alpha / beta R1 PerCP-conjugated Antibody

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source.

For research use only

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