Human IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11127
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1020369
Product Specifications
Immunogen
CHO-expressed recombinant Human IFN-beta protein
Met22-Asn187
Accession # P01574
Met22-Asn187
Accession # P01574
Specificity
Detects human INFB in direct ELISAs
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IFN-beta Antibody
Neutralization of IFN beta Anti-Viral Activity by Human IFN beta Antibody
Recombinant Human IFN beta ( 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC). Inhibition of anti-viral activity elicited by Recombinant Human IFN beta (50 pg/mL) is neutralized by Human IFN beta Antibody (Catalog # MAB11127). The ND50 for this effect is typically 2.00-20.0 µg/mL.Applications for Human IFN-beta Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 2-20 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-beta
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
UniProt
Additional IFN-beta Products
Product Documents for Human IFN-beta Antibody
Product Specific Notices for Human IFN-beta Antibody
For research use only
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