Human Ig Kappa Light Chain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10050
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 999916
Product Specifications
Immunogen
Human Ig Kappa Light Chain
Specificity
Detects Human Ig Kappa Light Chain in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Ig Kappa Light Chain Antibody
Detection of Human Ig Kappa Light Chain by Western Blot.
Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Ig Kappa Light Chain by Simple WesternTM.
Simple Western lane view shows lysates of human kappa light chain protein, human lambda light chain protein (negative control), and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Kappa Light Chain at approximately 32 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human Ig Kappa Light Chain ELISA Standard Curve.
Human Ig Kappa Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Ig Kappa Light Chain Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Simple Western
20 µg/mL
Sample: Human kappa light chain protein and Human serum
Sample: Human kappa light chain protein and Human serum
Western Blot
2 µg/mL
Sample: Human kappa light chain protein and Human serum
Sample: Human kappa light chain protein and Human serum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ig Kappa Light Chain
The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa) or a lambda ( lambda) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.
Long Name
Immunoglobulin Kappa Light Chain
Alternate Names
ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC
Additional Ig Kappa Light Chain Products
Product Documents for Human Ig Kappa Light Chain Antibody
Product Specific Notices for Human Ig Kappa Light Chain Antibody
For research use only
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