Human IGF-I/IGF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB291
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, ELISA Development (Capture), ELISA Microarray Development, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 56408
Product Specifications
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343
Gly49-Ala118
Accession # P01343
Specificity
Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IGF-I/IGF-1 Antibody
Detection of Recombinant Human IGF-I/IGF-1 by Western Blot.
Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human IGF-I/IGF-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Human IGF-I/IGF-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB291 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
UniProt
Additional IGF-I/IGF-1 Products
Product Documents for Human IGF-I/IGF-1 Antibody
Product Specific Notices for Human IGF-I/IGF-1 Antibody
For research use only
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