Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IGFLR1 Monoclonal Antibody (Catalog # MAB8296) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human IGFLR1 Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.3 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of rhIGFL-1 to immobilized rhIGFLR1 coated at 100 ng/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFLR1

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL-1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL-1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

References

Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.

Long Name

IGF-like Family Receptor 1

Alternate Names

TMEM149, U2AF1L4

Entrez Gene IDs

79713 (Human); 101883 (Mouse); 499126 (Rat)

Gene Symbol

IGFLR1

UniProt

Q9H665

Additional IGFLR1 Products

Product Documents for Human IGFLR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IGFLR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IGFLR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8296

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IGFLR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human IGFLR1 Antibody

Detection of IGFLR1 in Human Blood Lymphocytes by Flow Cytometry.