Human IgG4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9895
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Neutralization Control
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 985547
Product Specifications
Immunogen
Purified IgG4 from normal human plasma
Specificity
Detects human IgG4 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human IgG4 Antibody
Detection of Human IgG4by Western Blot.
Western blot shows lysates of purified human IgG1(negative control), purified human IgG2(negative control), purified human IgG3(negative control), and human purified human IgG4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG1at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Human IgG4ELISA Standard Curve.
Recombinant Human IgG4protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human IgG4 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Western Blot
2 µg/mL
Sample: Purified human IgG4
under non-reducing conditions only
Sample: Purified human IgG4
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG4
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G4
Alternate Names
Immunoglobulin G, Immunoglobulin G4, ImmunoglobulinG
Additional IgG4 Products
Product Documents for Human IgG4 Antibody
Product Specific Notices for Human IgG4 Antibody
For research use only
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