Western blot shows lysates of TT human medullary thyroid cancer cell line and human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human IGSF8/CD316 Monoclonal Antibody (Catalog # MAB3117) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for IGSF8/CD316 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IGSF8 in SHSY-5Y human cell line by Flow Cytometry.

SHSY-5Y human neuroblastoma cell line was stained with Rabbit Anti-Human IGSF8 Monoclonal Antibody (Catalog # MAB3117, filled histogram) or Rabbit IgG isotype control antibody (Catalog # MAB1050, open histogram) followed by Anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111). View our protocol for Staining Membrane-associated Proteins.

Detection of Human IGSF8/CD316 by Simple Western^TM.

Simple Western lane view shows lysates of Human Brain (Cerebellum), loaded at 0.2 mg/mL. A specific band was detected for IGSF8/CD316 at approximately 73 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human IGSF8/CD316 Monoclonal Antibody (Catalog # MAB3117) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IGSF8/CD316 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: SHSY-5Y human neuroblastoma cell line

Simple Western

20 µg/mL
Sample: Human Brain (Cerebellum)

Western Blot

2 µg/mL
Sample: TT human medullary thyroid cancer cell line and Human brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF8/CD316

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha3 beta1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha-Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

References

Clark, K.L. et al. (2001) J. Immunol. 167:5115.
Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
Charrin, S. et al. (2003) Biochem. J. 373:409.
Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
Kolesnikova, T. et al. (2009) Neoplasia.11:77.

Long Name

Immunoglobulin Superfamily, Member 8

Alternate Names

CD316, CD81P3, EWI2, KCT4, PGRL

Entrez Gene IDs

93185 (Human); 140559 (Mouse)

Gene Symbol

IGSF8

UniProt

Q969P0

Additional IGSF8/CD316 Products

Product Documents for Human IGSF8/CD316 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IGSF8/CD316 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IGSF8/CD316 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3117

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IGSF8/CD316 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IGSF8/CD316 Antibody

Detection of Human IGSF8/CD316 by Western Blot.