Human IkB-epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4300
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 418515
Product Specifications
Immunogen
E. coli-derived recombinant human IkB-epsilon
Ser64-Asp423
Accession # Q96F31
Ser64-Asp423
Accession # Q96F31
Specificity
Detects endogenous human and mouse IkB-epsilon in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IkB-epsilon Antibody
Detection of Human IkB-epsilon by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line, PC-3 human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human IkB-e Monoclonal Antibody (Catalog # MAB4300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-e at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human IkB-epsilon Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: DA3 mouse myeloma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line
Sample: DA3 mouse myeloma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-epsilon
NF kappaB inhibitor epsilon (IkB epsilon) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB epsilon degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.
Long Name
I-kappa-B-epsilon
Alternate Names
IkBepsilon, NFKBIE
Gene Symbol
NFKBIE
UniProt
Additional IkB-epsilon Products
Product Documents for Human IkB-epsilon Antibody
Product Specific Notices for Human IkB-epsilon Antibody
For research use only
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