Human IkB-epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4300
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IkB-epsilon
Ser141-Asp500
Accession # O00221
Ser141-Asp500
Accession # O00221
Specificity
Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IkB-epsilon Antibody
IkB‑ epsilon in Human Astrocytoma.
IkB-e was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human IkB-epsilon Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human astrocytoma
Sample: Immersion fixed paraffin-embedded sections of human astrocytoma
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-epsilon
NF kappaB inhibitor epsilon (IkB-epsilon) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.
Long Name
I-kappa-B-epsilon
Alternate Names
IkBepsilon, NFKBIE
Gene Symbol
NFKBIE
UniProt
Additional IkB-epsilon Products
Product Documents for Human IkB-epsilon Antibody
Product Specific Notices for Human IkB-epsilon Antibody
For research use only
Loading...
Loading...
Loading...
Loading...