Human IL-10 R alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB274
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Blocking, Flow Cytometry, Functional Assay, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 37607
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 R alpha
His22-Asn235
Accession # Q13651.1
His22-Asn235
Accession # Q13651.1
Specificity
Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-10 R alpha Antibody
IL-10 Inhibition of IL-1 beta secretion and Neutralization by Human IL-10 R alpha Antibody.
Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta/IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-10 Ra Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).Applications for Human IL-10 R alpha Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL-10 R alpha-mediated IL-10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL-10 and 0.25 ng/mL LPS.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10 R alpha
IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.
Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.
Long Name
Interleukin 10 Receptor alpha
Alternate Names
CDw210a, IL-10Ra, IL10R alpha, IL10RA
Gene Symbol
IL10RA
UniProt
Additional IL-10 R alpha Products
Product Documents for Human IL-10 R alpha Antibody
Product Specific Notices for Human IL-10 R alpha Antibody
For research use only
Loading...
Loading...
Loading...