Human IL-11 R alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1977

R&D Systems, part of Bio-Techne
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MAB1977
MAB1977-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 473143

View all IL-11 R alpha Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-11 R alpha
Cys26-Val363
Accession # Q14626

Specificity

Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IL-11 R alpha Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL-11 R alpha Fc Chimera (Catalog # 1977-MR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11 R alpha

Human interleukin 11 receptor alpha (IL-11 R alpha) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha, respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

References

  1. Cherel, M. et al. (1995) Blood 86:2534.
  2. Schleinkofer, K. et al. (2001) J. Mol. Biol. 306:263.
  3. Nandurkar, H.H. et al. (1996) Oncogene 12:585.
  4. Taga, T. et al. (1997) Annu. Rev. Immuno. 15:797.
  5. Davidson, A.J. et al. (1997) Stem Cells 15:119.
  6. Curtis, D.J. et al. (1997) Blood 90:4403.

Long Name

Interleukin 11 Receptor alpha

Alternate Names

IL-11Ra, IL11R alpha, IL11RA

Entrez Gene IDs

3590 (Human); 16157 (Mouse)

Gene Symbol

IL11RA

UniProt

Q14626

Additional IL-11 R alpha Products

Product Documents for Human IL-11 R alpha Antibody

Product Datasheets

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Product Specific Notices for Human IL-11 R alpha Antibody

For research use only

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