Human IL-12 R beta1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB839P
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Cys24-Glu540
Accession # P42701
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human IL-12 R beta1 PE-conjugated Antibody
Detection of IL‑12 R beta1 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 µg/mL PHA for 48 hours or (B) untreated were stained with Mouse Anti-Human IL-12 R beta1 PE-conjugated Monoclonal Antibody (Catalog # FAB839P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.Detection of IL-12 R beta1 in Daudi cells by Flow Cytometry
Daudi cells were stained with Mouse Anti-Human IL-12 R beta1 PE-conjugated Monoclonal Antibody (Catalog # FAB839P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human IL-12 R beta1 PE-conjugated Antibody
Flow Cytometry
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA; Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1
Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta1 and IL-12 R beta2. Individually, IL-12 R beta1 and IL-12 R beta2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta2 is more restricted and appears to be limited to Th2 cells.
References
- Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
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Additional IL-12 R beta 1 Products
Product Documents for Human IL-12 R beta1 PE-conjugated Antibody
Product Specific Notices for Human IL-12 R beta1 PE-conjugated Antibody
For research use only