Human IL-12 R beta2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB19591
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 622509
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta2
Lys24-Cys622
Accession # Q99665
Lys24-Cys622
Accession # Q99665
Specificity
Detects human IL-12 R beta2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL‑12 R beta1 or recombinant mouse IL-12 R beta1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human IL-12 R beta2 Antibody
Detection of Human IL‑12 R beta2 by Western Blot.
Western blot shows lysates of human CD4+in vitrodifferentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL-12 R beta2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human Th1 cells
Sample: Human Th1 cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 R beta 2
IL-12 R beta2 is a type I cytokine receptor family protein that associates with IL 12 R beta1 to form a functional high affinity receptor complex for IL-12. The extracellular region of IL 12 R beta2 contains five fibronectin type 3 domains. The cytoplasmic region is alternatively spliced, giving rise to two isoforms. IL-12 R beta2 is up‑regulated in Th1 cells during inflammatory responses. The extracellular regions of human and mouse IL‑12 R beta2 share 69% amino acid sequence identity.
Long Name
Interleukin 12 Receptor beta 2
Alternate Names
IL-12Rb2, IL12R beta 2, IL12RB2
Gene Symbol
IL12RB2
UniProt
Additional IL-12 R beta 2 Products
Product Documents for Human IL-12 R beta2 Antibody
Product Specific Notices for Human IL-12 R beta2 Antibody
For research use only
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