Human IL-13 R alpha2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB614
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Development, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 83834
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-13 R alpha2
Cys22-Leu342
Accession # Q14627
Cys22-Leu342
Accession # Q14627
Specificity
Detects human IL-13 R alpha2 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human (rh) IL‑4 R and rhIL‑9 R and no cross-reactivity with rhIL‑5 R alpha, rhIL‑5 R beta, rhIL‑13 R alpha1, or recombinant mouse IL‑13 R alpha2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-13 R alpha2 Antibody
Detection of Human IL‑13 R alpha2 by Western Blot.
Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-13 Ra2 Monoclonal Antibody (Catalog # MAB614) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL-13 R alpha2 Antibody
Application
Recommended Usage
Western Blot
5 µg/mL
Sample: Human placenta tissue and human testis tissue
Sample: Human placenta tissue and human testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 2
Two type I membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with high affinity. The lower affinity IL-13 binding protein is now referred to as IL-13 R alpha1 and is also known as CD213a. IL-13 R alpha1 combines with IL-4 R alpha to form a high affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The higher affinity IL-13 binding protein, now referred to as IL-13 R alpha2, does not induce a signal and acts as a decoy receptor.
Long Name
Interleukin 13 Receptor alpha 2
Alternate Names
CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2
Gene Symbol
IL13RA2
UniProt
Additional IL-13 R alpha 2 Products
Product Documents for Human IL-13 R alpha2 Antibody
Product Specific Notices for Human IL-13 R alpha2 Antibody
For research use only
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