Human IL-17/IL-17A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3171R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 41802R
Product Specifications
Immunogen
E.coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer and no cross-reactivity with rhIL-17F, recombinant mouse IL-17, and recombinant rat IL-17A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-17/IL-17A Antibody
Detection of Human IL‑17/IL‑17A by Western Blot.
Western blot shows lysates of human CD4+T cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-17/IL-17A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IL‑17/IL‑17A by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-17/IL-17A. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-17/IL-17A at approximately 14-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL calcium ionomycin for 16 hours, then stained with Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-17/IL-17A Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
1 µg/mL
Sample: Human CD4+ T cells or HEK293 human embryonic kidney cell line transfected human IL-17/IL-17A
Sample: Human CD4+ T cells or HEK293 human embryonic kidney cell line transfected human IL-17/IL-17A
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5
mg/mL in sterile PBS.
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
UniProt
Additional IL-17/IL-17A Products
Product Documents for Human IL-17/IL-17A Antibody
Product Specific Notices for Human IL-17/IL-17A Antibody
For research use only
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