Human IL-18/IL-1F4 Propeptide Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC646R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 74801
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑18/IL‑1F4
Accession # Q14116
Accession # Q14116
Specificity
Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross‑react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-18/IL-1F4 Propeptide Alexa Fluor® 647-conjugated Antibody
Detection of IL-18/IL-1F4 Propeptide in Human THP-1 cells by Flow Cytometry.
THP-1 human acute monocytic leukemia cells were stained with Mouse Anti-Human IL-18/IL-1F4 Propeptide Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC646R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-18/IL-1F4 Propeptide Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: THP-1 human acute monocytic leukemia cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18/IL-1F4
Pro-IL-18 (pro-Interleukin 18; also pro-IGIF and pro-IL-1 gamma) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta-subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.
Long Name
Interleukin 18
Alternate Names
IGIF, IL-1F4, IL-1g, IL18
Gene Symbol
IL18
UniProt
Additional IL-18/IL-1F4 Products
Product Specific Notices for Human IL-18/IL-1F4 Propeptide Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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