Human IL-18 R beta/IL-1 R7 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB118R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 132029
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta/IL‑1 R7
Met1-Arg356
Accession # O95256
Met1-Arg356
Accession # O95256
Specificity
Detects human IL‑18 R beta/IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL‑1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity with rhIL-1 RI, rhIL‑1 RacP/IL‑1 R3, rhIL-1 R4, rhIL-18 R alpha/IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta/IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta is observed and no cross-reactivity with rhIL-18 R alpha/IL-1 R5, rhIL‑1 RacP/IL‑1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human IL-18 R beta/IL-1 R7 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: KG-1 human acute myelogenous leukemia cell line
Sample: KG-1 human acute myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-18 R beta/IL-1 R7
IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
References
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Interleukin 18 Receptor beta
Alternate Names
AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP
Gene Symbol
IL18RAP
UniProt
Additional IL-18 R beta/IL-1 R7 Products
Product Documents for Human IL-18 R beta/IL-1 R7 Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Human IL-18 R beta/IL-1 R7 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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