Human IL-1 alpha/IL-1F1 (Research Grade Bermekimab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10568
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu146
Product Specifications
Immunogen
Human IL‑1 alpha/IL‑1F1
Specificity
Detects human IL‑1 alpha/IL‑1F1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Bermekimab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images
Detection of IL‑1 alpha /IL‑1F1 (Bermekimab) in LPS-treated Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells were (A) treated overnight with 1 µg/mL LPS or (B) untreated, then stained with Human IL-1a/IL-1F1 (Bermekimab) Monoclonal Antibody (Catalog # MAB10568, filled histogram) or unstained (open histogram), followed by Anti-Human IgG Allophycoerythrin-conjugated Secondary Antibody (F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.Applications
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: LPS-treated Human PBMC
Sample: LPS-treated Human PBMC
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Specific Notices
For research use only
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