Human IL-21 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF15001

R&D Systems, part of Bio-Techne
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AF15001-100
AF15001-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-21 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-21
Gln25-Ser155
Accession # Q9HBE4

Specificity

Detects human IL-21 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-21 Antibody

Detection of Human IL-21 antibody by Western Blot.

Detection of Human IL‑21 by Western Blot.

Western blot shows lysates of human CD4+ T cells (negative control) and human CD4+ Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15001) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-21 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human IL-21 Antibody in ELISA Standard Curve.

Human IL‑21 ELISA Standard Curve.

Recombinant Human IL-21 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-21 Monoclonal Antibody(Catalog # MAB1500) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15001) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IL-21 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑21 Monoclonal Antibody(Catalog # MAB1500).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-21 DuoSet ELISA Kit (Catalog # DY8879-05) for convenient development of a sandwich ELISA.

Western Blot

1 µg/mL
Sample: Human CD4+ Th17 cells

Reviewed Applications

Read 1 review rated 4 using AF15001 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21

Interleukin-21 (IL-21) is an approximately 14 kDa four-helix-bundle member of the family of cytokines that utilize the common gamma chain ( gammac) as a receptor subunit. gammac is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (1). IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2-6). It exerts its biological effects through a heterodimeric receptor complex of gammac and the IL-21-specific IL-21 R (2, 7). Tfh-derived IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL-21 regulates several aspects of T cell function. It co-stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells (13, 14). IL-21 R engagement enhances the cytolytic activity and IFN-gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL-21/IL-21 R system contributes to the development of multiple immunological disorders (1, 17). The 133 amino acid (aa) mature human IL-21 shares 63% and 61% aa sequence identity with mouse and rat IL-21, respectively. Alternative splicing generates an additional isoform with a substitution of the C-terminal 16 amino acids (18).

References

  1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
  2. Parrish-Novak, et al. (2000) Nature 408:57.
  3. Coquet, J.M. et al. (2007) J. Immunol. 178:2827.
  4. Vogelzang, A. et al. (2008) Immunity 29:127.
  5. Korn, T. et al. (2007) Nature 448:484.
  6. Nurieva, R. et al. (2007) Nature 448:480.
  7. Asao, H. et al. (2001) J. Immunol. 167:1.
  8. Zotos, D. et al. (2010) J. Exp. Med. 207:365.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  11. Frohlich, A. et al. (2009) Science 324:1576.
  12. Yi, J.S., et al. (2009) Science 324:1572.
  13. Peluso, I. et al. (2007) J. Immunol. 178:732.
  14. Bucher, C. et al. (2009) Blood 114:5375.
  15. Kasaian, M.T. et al. (2002) Immunity 16:559.
  16. Tamagawa-Mineoka, R. et al. (2011) J. Invest. Dermatol. 131:1513.
  17. Ma, J. et al. (2011) Cytokine 56:133.
  18. Rahman, M. et al. (2007) FEBS Lett. 581:4001.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Entrez Gene IDs

59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

Gene Symbol

IL21

UniProt

Q9HBE4

Additional IL-21 Products

Product Documents for Human IL-21 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-21 Antibody

For research use only

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