Human IL-21R Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9911G
Clone 152512 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 152512
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R
Cys20-Pro236
Accession # Q9HBE5
Cys20-Pro236
Accession # Q9HBE5
Specificity
Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human IL-21R Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Human whole blood CD19+ B cells
Sample: Human whole blood CD19+ B cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-21R
Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma chain ( gammac) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21 Receptor
Alternate Names
CD360, IL-21 R, IL21R, NILR
Entrez Gene IDs
Gene Symbol
IL21R
UniProt
Additional IL-21R Products
Product Documents for Human IL-21R Alexa Fluor® 488-conjugated Antibody
Product Specific Notices for Human IL-21R Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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