Human IL-21R Antibody
R&D Systems, part of Bio-Techne | Catalog # AF991
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Functional Assay, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑21 R Subunit
Cys20-Pro236
Accession # Q9HBE5
Cys20-Pro236
Accession # Q9HBE5
Specificity
Detects human IL‑21 R Subunit in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse IL‑21 R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human IL-21R Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood CD19+ B cells
Sample: Human whole blood CD19+ B cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-21 R Subunit Fc Chimera (Catalog # 991-R2)
Sample: Recombinant Human IL-21 R Subunit Fc Chimera (Catalog # 991-R2)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21R
Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells and cell lines of T, B and NK lineage. The common gamma chain ( gammac) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21 Receptor
Alternate Names
CD360, IL-21 R, IL21R, NILR
Entrez Gene IDs
Gene Symbol
IL21R
UniProt
Additional IL-21R Products
Product Documents for Human IL-21R Antibody
Product Specific Notices for Human IL-21R Antibody
For research use only
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