Human IL-22 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7822
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Development, ELISA Development (Capture), ELISpot Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 142906
Product Specifications
Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Ala34-Ile179
Accession # Q9GZX6
Specificity
Detects human IL-22 in direct ELISAs and Western blots. Shows 100% cross‑reactivity with recombinant mouse (rm) IL-22 and recombinant rat IL-22 and no cross-reactivity with recombinant human IL-10. In sandwich ELISAs, no cross-reactivity was observed with rmIL-22, rrIL-22, rhIL-10, rhIL-22BP, or rhIL-22 R.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human IL-22 Antibody
Human IL-22 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB7822 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate
STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and
IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
References
- Dumoutier, L. et al., (2000) J. Immunol. 164:1814.
- Xie, M-H. et al., (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al., (2000) PNAS 97:10144.
- Kotenko, S.V. et al., (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Alternate Names
IL-TIF, IL22
Gene Symbol
IL22
UniProt
Additional IL-22 Products
Product Documents for Human IL-22 Antibody
Product Specific Notices for Human IL-22 Antibody
For research use only
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