Human IL-22 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF782
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Neutralization, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Ala34-Ile179
Accession # Q9GZX6
Specificity
Detects human, recombinant mouse, and recombinant rat IL-22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IL-10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-22 Antibody
Detection of Human IL‑22 by Western Blot.
Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL-22 in Human PBMCs.
IL-22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL‑22 Antibody.
Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND50 is typically 0.5-2.5 µg/mL.
Applications for Human IL-22 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS)
Western Blot
1 µg/mL
Sample: Human tonsil tissue and human breast cancer tissue
Sample: Human tonsil tissue and human breast cancer tissue
Neutralization
Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line [Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679]. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
References
- Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
- Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Alternate Names
IL-TIF, IL22
Gene Symbol
IL22
UniProt
Additional IL-22 Products
Product Documents for Human IL-22 Antibody
Product Specific Notices for Human IL-22 Antibody
For research use only
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