Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with PMA and Calcium ionomycin, then stained with Mouse Anti-Human IL-26/AK155 Monoclonal Antibody (Catalog # MAB13751, dark orange filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human IL-26/AK155 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-26/AK155

IL-26, also named AK155, belongs to the family of IL-10-related cytokines.

Long Name

Interleukin 26

Alternate Names

AK155, IL26

Entrez Gene IDs

55801 (Human)

Gene Symbol

IL26

UniProt

Q9NPH9.1

Additional IL-26/AK155 Products

Product Documents for Human IL-26/AK155 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-26/AK155 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-26/AK155 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13751

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-26/AK155 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-26/AK155 Antibody

Detection of IL-26/AK155 in Human PBMCs by Flow Cytometry.