Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of IL‑28 R alpha/IFN-lambda R1 in PBMCs by Flow Cytometry.

Peripheral blood mononuclear cells (PBMCs) either unstimulated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human IL-28 Ra/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-28 R alpha/IFN-lambda R1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Daudi human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN-lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Long Name

Interleukin 28 Receptor alpha

Alternate Names

CRF2-12, IFN-lambda R1, IFNLR1, IL-28Ra, IL28R alpha, IL28RA, LICR2

Entrez Gene IDs

163702 (Human); 242700 (Mouse); 362628 (Rat)

Gene Symbol

IFNLR1

UniProt

Q8IU57 (Human)

Additional IL-28 R alpha/IFN-lambda R1 Products

Product Documents for Human IL-28 R alpha/IFN-lambda R1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-28 R alpha/IFN-lambda R1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-28 R alpha/IFN-lambda R1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5260

Key Product Details

Validated by

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all IL-28 R alpha/IFN-lambda R1 Antibodies »

Product Summary for Human IL-28 R alpha/IFN-lambda R1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-28 R alpha/IFN-lambda R1 Antibody

Detection of IL‑28 R alpha/IFN-lambda R1 in Daudi Human Cell Line by Flow Cytometry.